Investigation of two different size microplastic degradation ability of thermophilic bacteria using polyethylene polymers

Akarsu C., ÖZDEMİR S., Ozay Y., Acer O., DİZGE N.

ENVIRONMENTAL TECHNOLOGY, cilt.44, sa.24, ss.3710-3720, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 24
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/09593330.2022.2071638
  • Dergi Adı: ENVIRONMENTAL TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, EMBASE, Environment Index, Geobase, Greenfile, INSPEC, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, DIALNET, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.3710-3720
  • Anahtar Kelimeler: Biodegredation, microplastic degradation, polyethylene, thermophilic bacteria, LOW-DENSITY POLYETHYLENE, WATER TREATMENT-PLANT, WASTE-WATER, MICROBIAL-DEGRADATION, FRESH-WATER, BIODEGRADATION, POLYPROPYLENE, PLASTICS, FATE, QUANTIFICATION
  • İstanbul Üniversitesi Adresli: Hayır

Özet

There are several studies stating that many types of microplastics cannot be retained completely by conventional wastewater treatment systems. Therefore, it is necessary to prevent the discharge of these microplastics to the ecological system. The objective of this study was to investigate the biodegradation ability of two different size of PE (50 and 150 mu m) by using two Gram-positive, spore-forming, rod-shaped, and motile thermophilic bacteria, called strain Gecek4 and strain ST5, which can hydrolyse starch, were isolated from the soil's samples of Gecek and omer hot-springs in Afyonkarahisar, Turkey, respectively. Phenotypic features and 16S rRNA analyzing of strains also studied. According to these results, Gecek4s and ST5 were identified as Anoxybacillus flavithermus Gecek4s and Bacillus firmus ST5, respectively. Results showed that A. flavithermus Gecek4s could colonise the polymer surface and cause surface damage whereas B. firmus ST5 could not degrade bigger-sized particles efficiently. In addition, morphological changes on microplastic surface were investigated by scanning electron microscopy (SEM) where dimensional changes, irregularities, crack, and/or holes were detected. This finding suggests that there is a high potential to develop an effective integrated method for plastic bags degradation by extracellular enzymes from bacteria.