Survival of Biofilm-Associated Legionella pneumophila Exposed to Various Stressors

Vatansever C., Turetgen I.

WATER ENVIRONMENT RESEARCH, cilt.87, sa.3, ss.227-232, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 87 Sayı: 3
  • Basım Tarihi: 2015
  • Doi Numarası: 10.2175/106143015x14212658613154
  • Dergi Adı: WATER ENVIRONMENT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.227-232
  • Anahtar Kelimeler: biofilm, exposure, heat stress, Legionella pneumophila, monochloramine, PSEUDOMONAS-AERUGINOSA, WATER, RESISTANCE, CHLORINE, BACTERIA, EFFICACY, GROWTH, CELLS, MODEL, MONOCHLORAMINE
  • İstanbul Üniversitesi Adresli: Evet

Özet

Biofilm is crucial for the multiplication and survival of Legionella pneumophila. The survival after different stressors of biofilm-associated L. pneumophila was evaluated during 150 days in this study. Mixed biofilms were allowed to develop on coupons in a biofilm reactor, which was experimentally infected with L. pneumophila. A dose of 2 ppm of monochloramine was found ineffective to kill younger (60 days) biofilm-associated L. pneumophila, whereas shock treatment (500 and 1000 ppm) was found to be significantly successful, as expected. Also, short exposure to 60 degrees C was insufficient to kill all young L. pneumophila within biofilms. A significant amount of young L. pneumophila bacteria also resisted pH 11 and 3 molar salt solution. No significant change was observed after exposure to 4 degrees C, ultra pure water and pH 5. Interestingly, L. pneumophila bacteria in biofilm became more sensitive after 90 days.