Efficacy of fipronil, which until now has not been assessed in Turkey, was tested against fleas (Ctenocephalides felis, Ct. canis) on cats and fleas (Ct. canis, Ct. felis, Pulex irritans) and ticks (Rhipicephalus sanguineus) on dogs. Fipronil spot-on (Spotline (R), TOPKIM Ilac Sanayi AS, Turkey) was applied directly on to the two different skin points between the shoulder blades of animals at recommended practical doses based on the dose of 0.067 ml/kg (6.7 mg active ingredient/kg). Animals were examined for the presence of parasites several days (3-5 days) after treatment. Drug efficacy was calculated from the percentages of animals becoming free of parasites after treatment. Fipronil was 100% effective to remove and/or kill fleas on both dogs (16 dogs) and cats (15 cats) and ticks on dogs (12 dogs). Live and death parasites in decreasing numbers as a result of re-infestation and after dying were seen on a dog for 9 days after treatment, which was put together with heavy flea infested puppies. No adverse reactions were observed in both dogs and cats treated.