4th EURASIA BIOCHEMICAL APPROACHES & TECHNOLOGIES (EBAT), Antalya, Turkey, 3 - 06 November 2022, pp.70
Some radionuclides used in nuclear medicine can be combined (radiolabeled) with molecules that are selective by cancerous or infected tissues, for the development of targeted drugs, as well as image functionality, and can be directed to these tissues.
Antibiotics have been used for many years in the treatment of infectious diseases. However, as a result of the widespread use of wrong and unnecessary antibiotics in the world and in our country, there is a rapid increase in antibiotic resistance. For this reason, innovative approaches are needed especially against microorganisms with biofilm structure and in the diagnosis and treatment of infectious diseases. One of these innovative approaches is "alternative to today's synthetic antibiotics, isolation of organic antibiotics from cave bacteria and development of innovative agents for imaging the infected area".
The antimicrobial activity of isolated organic cipro (o-CIP) produced by Micrococcus luteus bacteria collected from the “Yarık Dudeni Cave” in Mersin, Turkey. After produced organic antibiotics by isolated bacterial species were determinated, the structural analysis was done by Liquid Chromatography Quadrupole Time-Of-Flight Mass Spectrometry (LC/QTOF/MS) and High Performance Liquid Chromatography (HPLC) Analysis. According to LC/QTOF/MS, there was three organic antibiotics (Levofloxacin, Clindamycin and Ciprofloxacin).
Within the scope of the study, the isolation of organic antibiotics produced by Micrococcus luteus from bacterial media, the isolated organic antibiotics against pathogenic microorganisms (Escherichia coli (ATCC 9637), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 39327), Vancomycin Resistant Enterococci (ATCC MP-1) , SCCmec Type MRSA (ATCC MP-2), Candida albicans (ATCC 10231)), radiolabeling of isolated organic antibiotics, antimicrobial activity of radiolabeled antibiotics on these pathogenic microorganisms were investigated.
Organic antibiotic were radiolabelled demonstrated higher yield than synthetic derivative. Quality control studies were examined using the TLRC method and it was determined that both substances remained stable for 240 minutes. The % binding efficiency of radiolabeled antibiotics on pathogenic microorganisms were determined and compared with each other.