The Effect Of S-Adenosylmethionine On Liver Lesions And Oxidative Stress Induced By A High Fat And High Cholesterol Diet

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Çevik A.

XXXI. National Congress of the Turkish Biochemical Society, İstanbul, Türkiye, 18 - 20 Aralık 2020, cilt.45, ss.58, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Cilt numarası: 45
  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.58
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • İstanbul Üniversitesi Adresli: Evet

Özet

THE EFFECT OF S-ADENOSYLMETHIONINE ON LIVER LESIONS AND OXIDATIVE STRESS INDUCED BY A HIGH FAT AND HIGH CHOLESTEROL DIET

Ilknur Bi̇ngul, Department of Medical Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey

OBJECTIVES: Steatosis makes liver susceptible to oxidative stress, inflammation, cytokines, and triggers the formation of NASH and advanced lesions. S-adenosylmethionine (SAM) is the main methyl group donor, has antioxidant and anti-inflammatory effects, and regulates phosphatidylcholine synthesis. It has been suggested that changes in hepatic SAM levels are related to NASH pathogenesis. However, experimental studies on effects of SAM in NASH are limited. Our study investigated changes in SAM levels in a high-fat and cholesterol (HFHC) diet-induced NASH model, and the effect of SAM in preventing NASH formation and progression.

MATERIALS and METHODS: Female guinea pigs under HFHC-diet for 6 weeks, were injected SAM (50 mg/kg; 5 times/week; intraperitoneally). Serum transaminases (ALT, AST), cholesterol, triglyceride, insulin resistance (HOMAIR), inflammatory cytokines (TNF- α, IL-6) levels were determined. Hepatic cholesterol, triglyceride, SAM, reactive oxygen species (ROS), malondialdehyde (MDA), diene conjugate (DC), protein carbonyl (PC), glutathione (GSH) and antioxidant power (FRAP) levels were determined.

RESULTS: HFHC-diet increased ALT (p=0.005), AST (p=0.000), TNF- α (p=0.024), cholesterol (p=0.004), triglyceride ((p=0.001), levels in serum, but HOMA-IR did not change. Hepatic levels of cholesterol (p=0.000), triglyceride (p=0.000), ROS (p=0.000), MDA (p=0.010), DC (p=0.000), PC (p=0.035), and SAM (p=0.004), FRAP (p=0.004) decreased significantly. SAM treatment during HFHC-diet lowered ALT (p=0.000), AST (p=0.005) activities, hepatic triglyceride (p=0.019), ROS (p=0.000), MDA (p=0.004), DC (p=0.018), PC (p=0.046), and increased SAM (p=0.025), GSH (p=0.002) and FRAP (p=0.006) levels.

CONCLUSIONS: SAM treatment may be effective in preventing NASH formation by suppression of steatosis and oxidative stress.

Keywords: S-Adenosylmethionine, Nonalcoholic Steatohepatitis, Oxidative Stress, Liver; Guinea Pigs