Oocyte retrivial from ovarian corteof woman undergoing ovarian cortical ti,ssue cryopreservation


KERVANCIOĞLU M. E. , KIRAL C., ÇEPNİ İ., DEMİRKIRAN F., ÖCAL P., GEZER A., ...More

4TH ISFP WORLD CONGRESS, Şangay, China, 13 - 15 November 2015, pp.189

  • Publication Type: Conference Paper / Full Text
  • City: Şangay
  • Country: China
  • Page Numbers: pp.189

Abstract

Background

Recent advances in cancer therapy have led to improvement in survival. Best way to keep women from gonadotoxic effects of cancer therapy is to freeze oocyte or ovarian cortex. Freezing of ovarian cortex is preferred by patients because of it is time-consuming, has hopeful preliminary results, return of cycles after transfer and for some religious reasons. Aim of this preliminary study is to investigate the factors affecting oocyte retrieval from ovarian cortex in women ovarian cortical tissue cryopreserved.

Methods

Ovarian cortical tissue were cryopreserved in 27 cases between August 2010 and August 2015. Indications in patients were breast cancer in 11, gynecologic malignancies in 6, hematologic malignancies in 4, and other malignancies in 6 cases. Ovarian cortical tissue was obtained by using laparoscopy in 14 cases and laparotomy in 13 cases. Oocyte was searched in all media which were used in all steps of ovarian cryopreservation and founded oocytes were collected.

Results

A total of 42 immature oocytes were obtained by laparotomy in 7 of 13 cases and 22 immature oocytes were obtained by laparoscopy in 6 of 14 cases. A total of 20 oocytes were obtained from 4 cases who were administered light follicular stimulation. The mean number of oocytes were obtained 3.4±1.7, 5.3±1.5, 6.2±3.0 in breast cancer, hematologic malignancies and in others respectively. Immature oocytes were different stages (a,b). Nondegenerate immature oocytes were cultured up to 60 hours (c,d). M2 oocytes were freezed with vitrification.

Conclusion

Recent advancements in in vitro maturation and oocyte vitrification provide great opportunity for OC and oocyte cryopreservation. Ovarian cortical tissue preparation medium should be investigated in detail and the oocytes obtained during preparation should be frozen directly or after in vitro maturation