Voltammetric behavior and determination of doxycycline in pharmaceuticals at molecularly imprinted and non-imprinted overoxidized polypyrrole electrodes

Gurler B., Ozkorucuklu S., KIR E.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.84, ss.263-268, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 84
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.jpba.2013.06.009
  • Dergi Adı: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.263-268
  • Anahtar Kelimeler: Cyclic voltammetry, Doxycycline, Molecularly imprinting, Overoxidation, Polypyrrole, PERFORMANCE LIQUID-CHROMATOGRAPHY, PENCIL GRAPHITE ELECTRODE, TETRACYCLINE ANTIBIOTICS, CAPILLARY-ELECTROPHORESIS, AMPEROMETRIC DETECTION, DEGRADATION-PRODUCTS, POLYMERS, BINDING, SEPARATION, RESIDUES
  • İstanbul Üniversitesi Adresli: Hayır

Özet

In this paper, electrochemical behavior of a member of tetracycline group compounds doxycyline (DC) was investigated in 20, 30, 40% (v/v) acetonitrile (ACN)-water binary mixture at the pH of 2.0-4.0 prepared Britton-Robinson (BR) buffers, by the differential pulse voltammetric method (DPV). Voltammetric determination of DC was carried out using molecularly imprinted (MIP) and non-imprinted polypyrrole (NIP) modified electrodes. Both types of electrode were prepared by the cyclic voltammetric method. The effect of the ACN-water ratio and pH, on the performance of the MIP electrode was investigated and optimized. The highest anodic signal of DC with MIP electrode was obtained in BR buffer solution was prepared in 30% (v/v) ACN-water at pH 2.0. The detection limit was determined as 4.35 x 10(-5) M (S/N = 3). The method was also applied for electrochemical determination of DC in pharmaceutical samples. The recovery factors for Tetradox (R) and Monodox (R) were found 96.30%, 61.18% respectively with the relative standard deviations 0.46 and 0.54. The results of experiments using two commercial drugs showed that the MIP electrode can be used as a sensor to determine DC in pharmaceuticals. (C) 2013 Elsevier B.V. All rights reserved.