Investigation of metallo-beta-lactamase producing strains of Pseudomonas aeruginosa and Acinetobacter baumannii by E-test, disk synergy and PCR

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Atıf İçin Kopyala

Aktas Z., Kayacan C. B.

SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, cilt.40, sa.4, ss.320-325, 2008 (SCI-Expanded)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 40 Sayı: 4
• Basım Tarihi: 2008
• Doi Numarası: 10.1080/00365540701704698
• Dergi Adı: SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES
• Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Sayfa Sayıları: ss.320-325
• İstanbul Üniversitesi Adresli: Evet

Özet

Carbapenem non-susceptible Pseudomonas aeruginosa and Acinetobacter baumannii strains were tested for the presence of metallo-beta-lactamases (MBLs) by EDTA-synergy screening. Imipenem hydrolysis was investigated by a bioassay and IMP-/VIM-encoding genes by PCR. No blaIMP/VIM related genes or imipenemase activity were detected although E-test found all strains as MBL-positive. Disk synergy tests with 0.5M EDTA determined 63.6-100%, while those with 0.1M EDTA detected 0-7.7% of isolates as MBL producers. Most strains were susceptible to EDTA. In conclusion, for MBL-screening purposes, EDTA-synergy results change with molarity of EDTA, but even if some false positives are encountered, 0.1M EDTA seems to be acceptable.