Clofoctol (o-CLO): isolation from cave environment, radioiodination and its antibiofilm effect

Karatay K. B., Rachid N. A., Güngör N., Aras O., Muftuler F. Z. B.

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, vol.334, no.9, pp.6773-6783, 2025 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 334 Issue: 9
  • Publication Date: 2025
  • Doi Number: 10.1007/s10967-025-10160-9
  • Journal Name: JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Analytical Abstracts, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, INSPEC, Metadex, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.6773-6783
  • Keywords: Cave bacteria, Clofoctol (CLO), Infectious diseases, Iodine-131 (131I)
  • Istanbul University Affiliated: Yes

Abstract

Antibiotics are commonly used worldwide to treat microbial infections. However, the prolonged overuse of antibiotics has given rise to antibiotic resistance. Innovative approaches are needed for the diagnosis and treatment of bacterial infections, especially those caused by biofilm-forming bacteria. One approach is to use organic antibiotics produced by cave bacteria as an alternative to synthetic antibiotics. We explored the antibiotic production potential of bacteria isolated from the third deepest cave in Turkey. Following the purification of the antibiotic clofoctol (CLO) from cave bacteria, minimal inhibitory concentrations of organic, radioactively, and inertly labelled CLO against Staphylococcus aureus were compared with minimal inhibitory concentrations of synthetic, radioactively, and inertly labelled CLO. The antibiofilm effects of antibiotics, as well as their labelled derivatives, were also determined. When the effect of radiolabeled antibiotics on the number of bacteria in the biofilm was examined, it was found that they exhibited more pronounced effects with increasing antibiotic concentrations. After 24 h of incubation, the bacterial numbers in the biofilm for radiolabeled antibiotic concentrations of 4 mu g/mL were determined to be 3.4 and 2.9 Log10 CFU/mL, respectively. When all experimental results were evaluated, it was observed that I-131-o-CLO is promising as a new strategic anti-biofilm agent.