Comparative study of follicular fluid and cultured granulosa cell steroid production

Irez T., Elibol F., Aksu M., Kaleli S.

10th World Congress of In Vitro Fertilization and Assisted Reproduction, Vancouver, Canada, 24 - 28 May 1997, pp.365-368 identifier

  • Publication Type: Conference Paper / Full Text
  • Volume:
  • City: Vancouver
  • Country: Canada
  • Page Numbers: pp.365-368
  • Istanbul University Affiliated: Yes


To determine the steroidogenic capacity of follicular and cultured granulosa cells, estradiol and progesterone productions were studied in 10 fertilized and 10 nonfertilized IVF-ET cases. After separation of granulosa cells, they were cultured in EBSS within 24 hours. Follicular fluid obtained from follicles with fertilized oocytes contained higher levels of estradiol (E2), progesterone (P) and P/E2 ratio although the differences were statistically nonsignificant. Cultured granulosa cells demonstrated higher numbers per mililiter in fertilized oocyte group in comparison with the nonfertilized ones (p<0.05). However, nonfertilized group showed higher estradiol and progesterone production per 10 million granulosa cells (p<0.05) while P/E2 production rate of cultured granulosa cells was higher than the P/E2 ratio of fertilized group (p<0.05). These results suggest that preovulatory granulosa cell steroid production in favor of progesterone may deteriorate fertilization.