THE CLASTOGENIC EFFECT OF PYRIMETHAMINE (DARAPRIM) ON HUMAN-CHROMOSOMES IN LYMPHOCYTE-CULTURES


EGELI U., ERDOGAN G.

CELL BIOLOGY AND TOXICOLOGY, cilt.7, ss.347-356, 1991 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 7 Konu: 4
  • Basım Tarihi: 1991
  • Doi Numarası: 10.1007/bf00124070
  • Dergi Adı: CELL BIOLOGY AND TOXICOLOGY
  • Sayfa Sayıları: ss.347-356

Özet

In this study the clastogenic effect of pyrimethamine (Daraprim), a folic acid antagonist used for the treatment of toxoplasmosis and malaria on human chromosomes, was investigated. Pyrimethamine was added to in vitro lymphocyte cultures at six different concentrations: 0.05 (normal therapeutic dose), 0.1, 0.2, 0.4, 0.8, and 1.6 mg/ml. No proliferation was observed in any of the cultures containing 1.6 mg/ml pyrimethamine. The results of the cytogenetic evaluations show that the frequency of breaks and gaps increase significantly in dose-dependent manner. Thus, pyrimethamine has a clastogenic effect on human chromosomes.