Research Information System
International Journal of Radiation Biology, vol.101, no.6, pp.572-580, 2025 (SCI-Expanded)
Objectives: The present study aims to investigate the changes in the expression levels of miR-675 and some selenoproteins (Sel K, Sel W, and Sel P) in patients with breast cancer, before and after radiotherapy. Subjects and methods: This study included 35 breast cancer patients who applied to the Department of Radiation Oncology for radiotherapy and 25 healthy female controls. miR-675 expressions were analyzed by using quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Western blot was conducted to determine Sel K, Sel W, and Sel P expression levels. Biochemical parameters and complete blood count were analyzed by autoanalyzer system and automatic cell counter, respectively. Results: Plasma Sel K protein expression levels were decreased in the after-radiotherapy group compared to the control and before-radiotherapy groups, but plasma miR-675 expression levels were lower in both before and after radiotherapy groups compared to the control group. Conclusion: Our findings showed that miR-675 and Sel K expression levels changed after radiotherapy in breast cancer patients. These changes may have the potential to be biomarkers for the effectiveness of radiotherapy in breast cancer.