DETECTION AND SPREAD OF OXA-48-PRODUCING KLEBSIELLA OXYTOCA ISOLATES IN ISTANBUL, TURKEY

Nazik H., Aydin S., Albayrak R., Bilgi E. A., Yildiz I., Kuvat N., ...More

SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH, vol.45, no.1, pp.123-129, 2014 (SCI-Expanded) identifier identifier identifier

Abstract

Southeast Asian J Trop Med Public Health. 2014 Jan;45(1):123-9.

Detection and spread of oxa-48-producing Klebsiella oxytoca isolates in Istanbul, Turkey.

Nazik H, Aydin S, Albayrak R, Bilgi EA, Yildiz I, Kuvat N, Kelesoglu FM, Kelesoglu FM, Pakaştiçali N, Yilmaz F, Ongen B.

Abstract

Five OXA-48 producing Klebsiella oxytoca strains isolated in April-July 2010 were analyzed. Antibiotic susceptibility tests were performed using disc diffusion method and VITEK 2 system. Carbapenemase activity was investigated using the Modified Hodge test. Beta-lactamase genes were detected by PCR and blaOXA-48 was sequenced. Genetic relatedness between K. oxytoca isolates was investigated by pulse-field gel electrophoresis (PFGE). Carbapenemase activity was detected in 5 isolates by Modified Hodge test. Although all strains were resistant to ertapenem and imipenem, only one strain was also resistant to meropenem. BlaOXA-48 in 4 isolates harbored 2 or 3 other ESBL types, namely, blaTEM, blaSHV, blaCTX-M, or blaVEB. PFGE revealed 3 different pulso-types among the K. oxytoca isolates. The presence of OXA-48 carbapenemase in other species of clinical isolates should also be considered.

Five OXA-48 producing Klebsiella oxytoca strains isolated in April-July 2010 were analyzed. Antibiotic susceptibility tests were performed using disc diffusion method and VITEK 2 system. Carbapenemase activity was investigated using the Modified Hodge test. Beta-lactamase genes were detected by PCR and bla(OXA-48) was sequenced. Genetic relatedness between K. oxytoca isolates was investigated by pulse-field gel electrophoresis (PFGE). Carbapenemase activity was detected in 5 isolates by Modified Hodge test. Although all strains were resistant to ertapenem and imipenem, only one strain was also resistant to meropenem. Bla(OXA-48) in 4 isolates harbored 2 or 3 other ESBL types, namely, bla(TEM), bla(SHV), bla(CTX-M) or bla(VEB). PFGE revealed 3 different pulso-types among the K. oxytoca isolates. The presence of OXA-48 carbapenemase in other species of clinical isolates should also be considered.