DETECTION AND SPREAD OF OXA-48-PRODUCING KLEBSIELLA OXYTOCA ISOLATES IN ISTANBUL, TURKEY


Nazik H., Aydin S., Albayrak R., Bilgi E. A. , Yildiz I. , Kuvat N., ...Daha Fazla

SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH, cilt.45, ss.123-129, 2014 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 45 Konu: 1
  • Basım Tarihi: 2014
  • Dergi Adı: SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH
  • Sayfa Sayıları: ss.123-129

Özet

Five OXA-48 producing Klebsiella oxytoca strains isolated in April-July 2010 were analyzed. Antibiotic susceptibility tests were performed using disc diffusion method and VITEK 2 system. Carbapenemase activity was investigated using the Modified Hodge test. Beta-lactamase genes were detected by PCR and bla(OXA-48) was sequenced. Genetic relatedness between K. oxytoca isolates was investigated by pulse-field gel electrophoresis (PFGE). Carbapenemase activity was detected in 5 isolates by Modified Hodge test. Although all strains were resistant to ertapenem and imipenem, only one strain was also resistant to meropenem. Bla(OXA-48) in 4 isolates harbored 2 or 3 other ESBL types, namely, bla(TEM), bla(SHV), bla(CTX-M) or bla(VEB). PFGE revealed 3 different pulso-types among the K. oxytoca isolates. The presence of OXA-48 carbapenemase in other species of clinical isolates should also be considered.

Southeast Asian J Trop Med Public Health. 2014 Jan;45(1):123-9.

Detection and spread of oxa-48-producing Klebsiella oxytoca isolates in Istanbul, Turkey.

Nazik HAydin SAlbayrak RBilgi EAYildiz IKuvat NKelesoglu FMKelesoglu FMPakaştiçali NYilmaz FOngen B.

Abstract

Five OXA-48 producing Klebsiella oxytoca strains isolated in April-July 2010 were analyzed. Antibiotic susceptibility tests were performed using disc diffusion method and VITEK 2 system. Carbapenemase activity was investigated using the Modified Hodge test. Beta-lactamase genes were detected by PCR and blaOXA-48 was sequenced. Genetic relatedness between K. oxytoca isolates was investigated by pulse-field gel electrophoresis (PFGE). Carbapenemase activity was detected in 5 isolates by Modified Hodge test. Although all strains were resistant to ertapenem and imipenem, only one strain was also resistant to meropenem. BlaOXA-48 in 4 isolates harbored 2 or 3 other ESBL types, namely, blaTEM, blaSHV, blaCTX-M, or blaVEB. PFGE revealed 3 different pulso-types among the K. oxytoca isolates. The presence of OXA-48 carbapenemase in other species of clinical isolates should also be considered.