Carbapenem resistance in Turkey: Repeat report on OXA-48 in Klebsiella pneumoniae and first report on IMP-1 beta-lactamase in Escherichia coli


Aktas Z. , Satana D. , Kayacan C., Ozbek B., Gurler N., Somer A. , ...More

AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, vol.6, no.17, pp.3874-3878, 2012 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 17
  • Publication Date: 2012
  • Doi Number: 10.5897/ajmr12.036
  • Journal Name: AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
  • Journal Indexes: Science Citation Index Expanded, CAB Abstracts, Veterinary Science Database
  • Page Numbers: pp.3874-3878
  • Keywords: OXA-48, IMP-1, carbapenemase, intensive care unit (ICU), OXACILLINASE-MEDIATED RESISTANCE, DISK SYNERGY, PLASMID, ENTEROBACTERIACEAE, IDENTIFICATION, SPREAD, PSEUDOMONAS, ISTANBUL, IMIPENEM, STRAINS

Abstract

Meropenem resistant Klebsiella pneumoniae and Escherichia coli isolates were analyzed. A total of 18 non-duplicate meropenem resistant K. pneumoniae and one E. coli isolates from hospitalized patients were analyzed by polymerase chain reaction (PCR) and DNA sequencing to detect the OXA-48, IMP, VIM, and SPM-1 genes. Susceptibilities to antimicrobials were determined using an agar dilution method and E-test; beta-lactamase production was detected by double disk synergy test [Imipenem, imipenem/ethylenediaminetetraacetic acid (EDTA)] (DDST), E-test MBL and E-test ESBL. Homology of the isolates was determined by randomly amplified polymorphic DNA typing (RAPD). Minimum inhibitory concentrations (MICs) of imipenem and meropenem were 2 to 8 and 4 to > 512 mg/L for K. pneumoniae; 2 and 32 mg/L for E. coli, respectively. The isolates were resistant to all beta-lactams. The DDST was positive only for the E. coli. PCR and sequencing identified bla(IMP-1) in the E. coli strain and bla(OXA-48) in all K. pneumoniae isolates. RAPD-PCR indicated that spread of OXA-48 producing K. pneumoniae in the pediatric intensive care unit (ICU) from June, 2007 to December, 2007 was clonal. This is the first report of IMP-1-producing E. coli in Turkey, while OXA-48 carbapenemases in K. pneumoniae have been reported recently from our country. Carbapenem resistance may spread among Enterobacteriaceae via the transferable enzyme OXA-48, as well as metallo-beta-lactamases.