© 2015 by Türkiye Klinikleri.The aim of the present study was to investigate new markers for metabolic syndrome. Fetuin-A is a physiological inhibitor of insulin receptor and is associated with insulin resistance. Adipocyte fatty acid-binding protein regulates cellular lipid metabolism by carrying free fatty acids to various intracellular compartments. Recently antioxidant and insulinotropic roles of adipocyte fatty acid-binding protein were determined. 8-hydroxydeoxyguanosine is as a marker of oxidative DNA damage. In the present study, plasma levels of fetuin A, adipocyte fatty acid-binding protein and 8-hydroxydeoxyguanosine were determined in cases with metabolic syndrome. Material and Methods: A total of 60 cases with metabolic syndrome were included in the study. The control group was constituted by age-matched 20 healthy volunteers. Fasting blood samples were taken from cases, plasma levels of fetuin-A, adipocyte fatty acid-binding protein and 8-hydroxydeoxyguanosine were measured with competitive ELISA kits. Results: Plasma levels of 8- hydroxydeoxyguanosine and adipocyte fatty acid-binding protein levels were found to be significantly higher in the cases with metabolic syndrome in comparison to those in the control group. No significant difference was determined between study groups for fetuin A level. Conclusion: Plasma level of 8-hydroxydeoxyguanosine and adipocyte fatty acid-binding protein are at a high level in cases with metabolic syndrome. High 8- hydroxydeoxyguanosine level may be considered as an evidence for increased cancer risk in cases with metabolic syndrome. In addition, it was concluded that high adipocyte fatty acid-binding protein level may be related with hyperinsulinemia.