Screening of Y chromosome microdeletion which contains AZF regions in 71 Turkish azoospermic men

Okutman-Emonts O., PEHLİVAN S., Tavmergen E., Tavmergen-Goker E., Ozkinay F.

GENETIC COUNSELING, cilt.15, sa.2, ss.199-205, 2004 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 2
  • Basım Tarihi: 2004
  • Dergi Adı: GENETIC COUNSELING
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.199-205
  • Anahtar Kelimeler: azoospermia factor (AZF), azoospermic men, microdeletion, Y chromosome, INTRACYTOPLASMIC SPERM INJECTION, MALE-INFERTILITY, LONG ARM, SEVERE OLIGOZOOSPERMIA, DAZ GENE, DELETIONS, TRANSMISSION, FERTILITY, FATHER, MALES
  • İstanbul Üniversitesi Adresli: Hayır

Özet

Screening of Y chromosome microdeletion which contains AZF regions in 71 turkish azoospermic men: In 71 Turkish men Y chromosome microdeletions have been studied before intracytoplasmic sperm injection (ICSI). DNA samples were amplified with 18 STS primers of the azoospermia factor (AZF) region on the Y chromosome by using multiplex polymerase chain reaction (PCR). Microdeletions were detected in 4 azoospermic men (5.6%); one with a deletion in the AZFb region, while the 3 others had a large deletion extending over multiple chromosomal regions (AZFb+c+d and AZFa+b+c+d). In the patients with microdeletion, no spermatogenetic activity could be detected in testis biopsies. This result confirms the idea that Y chromosome microdeletion analysis is important in investigating the possibility of finding sperm in testicular sperm extraction (TESE). Therefore, we point out the importance of genetic testing and counselling regarding Y chromosome microdeletion for couples requesting ICSI.