Background: The goal of this research is to develop a new electroanalytical procedure for the simultaneous electrochemical detection of vitamin K1 (VK1) and vitamin D3 (VD3), in a sodium dodecyl sulfate (SDS) -containing buffer solution, with no detectable interference of other kinds of vitamin A and vitamin E present in the sample solution. Surfactant solutions are well known their capability to dissolve oil-soluble substance such as dyes, hydrocarbons, esters, perfumes, and so on. Micellar solubilization is the powerful alternative for the dissolution of poorly soluble or hydrophobic compounds in aqueous environments. Surfactants micellar media can be successfully applied for the solubilization of lipophilic organic compounds in water media and enhancement of their electrochemical activity. In this research, the anionic surfactant SDS was used as the solubilizing agent.