Importance Of Polynnorphisms Of The MIF Gene At Acute Leukemia And Its Effect On Febrile Neutropenic Attacks
EUROPEAN JOURNAL OF THERAPEUTICS, vol.15, no.2, pp.5-9, 2009 (ESCI, TRDizin)
- Publication Type: Article / Article
- Volume: 15 Issue: 2
- Publication Date: 2009
- Journal Name: EUROPEAN JOURNAL OF THERAPEUTICS
- Journal Indexes: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
- Page Numbers: pp.5-9
- Keywords: Macrophage Migration Inhibitory Factor Gene, MIF-173 G/C polymorphism, PCR, Acute Leukemia, DNA
- Istanbul University Affiliated: Yes
Abstract
The objective of this study is to research polymorphisms in MIF gene -173 site in variable haemotological malignities and evaluate its effects on febrile neutropenic attacks after chemotherapy in acute leukemia, Single nucleotide polyrnorphisms (SNPs) a-173 site of the MIF gene was analysed with the PCR-RFLP method (restriction endonudease Alu I) with isolated DNA samples from 48 patients who were treated for ALL and AML and 53 healthy controls, and G and C allel frequencies were compared statistically between patients and healthy controls groups, As a result of the analyzis, it was observed that the rate of patients with GG genotypes was 70.9%, with GC genotypes 29.1%, and with CC genotypes 0%, Association is evaluated between MIF pozItivities in acute leukemias and distribution of infections, its factors, duration of feveral days and neutropenia. Within the GC allel group; no significant differences were observed (p>0.05) in infection factors, duration of neutropenia (<500/L), duration of feveral days, fungal pnomoni, blood infections and FN mortality compared to the GG allel group. In conclusion, fungal pneumonia was observed at a higher rate in the CG allel group (22%) cumpared to the GG allel group (11.5%); a repeat of the study with an expended group can yield more information about this relationship.