Ögret Y., Seda Akdemir A., Dogan S., Mart Komurcu S. Z., Damla Korkmaz M., Kaya E., ...Daha Fazla
Journal of Immunology, cilt.12, sa.1, ss.1-9, 2025 (Hakemli Dergi)
Özet
Objective: Various cellular pathologies in leukocytes contribute to the pathology of COVID-19 caused by SARS-CoV-2 infection. In this study, we
aimed to determine the prognostic significance of netotic and pyroptotic cell deaths in relation to disease severity in COVID-19 pathophysiology.
Materials and Methods: Serum samples were collected from a total of 150 patients with mild, moderate, and severe COVID-19, as defined by
the COVID-19 diagnosis and treatment guidelines, along with 89 healthy individuals. Circulating markers of netosis and pyroptosis, including
neutrophil elastase (NE), citrullinated histone H3 (CitH-H3), myeloperoxidase (MPO), cell-free DNA (cfDNA), gasdermin D (GSDMD), IL-18, and
IL-8, were analyzed using the ELISA method. The effects of netosis and pyroptosis markers on disease severity and clinical parameters were
comparatively evaluated, and their associations with inflammation, thrombosis, and fibrinolysis were also investigated.
Results: NE, GSDMD, IL-18, MPO, CITH3, D-dimer, leukocyte and lymphocyte levels, as well as inflammatory markers (CRP, ferritin), showed
a significantly elevated in severe COVİD-19 patients compare to the other groups.
Conclusion: Our findings indicate that circulating netosis and pyroptosis markers in SARS-CoV-2 infections correlate with inflammation,
dysregulated hemostasis, and fibrinolysis markers, playing a role in disease prognosis. These associations may provide potential therapeutic
targets for the treatment of severe COVID-19 cases.
Keywords : SARS-CoV-2, netosis, pyroptosis.