Investigation of HHV-8 infection in HIV infected people in Istanbul, Turkey

Yilmaz G., Ozkan E., Basaran P., Kucukbasmaci O.

14th International AIDS Conference, Barcelona, Spain, 7 - 12 July 2002, pp.43-47 identifier

  • Publication Type: Conference Paper / Full Text
  • Volume:
  • City: Barcelona
  • Country: Spain
  • Page Numbers: pp.43-47
  • Istanbul University Affiliated: Yes

Abstract

The most common neoplasm in AIDS patients is Kaposi's sarcoma (KS). Human herpesvirus 8 (HHV-8) is found to be responsible from KS. It is intended to investigate this newly identified virus in 50 HIV infected patients and 49 blood donors. For this purpose HHV-8 DNA was investigated in peripheral blood mononuclear cells by nested PCR with P1/P2 primers as the outher pair and P3/P4 sequences as the inner. pair. The predicted amplified, band was 233 basepairs. HHV-8 IgG antibodies was detected by IFA using Biotrin HHV-8 IgG IFA (Ireland).HHV-8 DNA was only detected in one HIV infected patient with KS. All blood donors were negative for HHV-8 DNA. HHV-8 IgG antibodies were found to be positive in 4 % and 21 % of blood donors and HIV seropositives, respectively.