Akademik Veri Yönetim Sistemi
5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022), Ankara, Türkiye, 23 - 25 Kasım 2022, ss.1729, (Özet Bildiri)
Cancer stem cells (CSCs) as tumor initiating cells are responsible for cancer reoccurrence and drug resistance and
differentiate into heterogeneous lineages of cancer cells. Information on the importance of the slight increase in Reactive
Oxygen Species (ROS) levels induced by low hydrogen peroxide (H2O2) in cancer stem cell biology is limited. We aimed
to investigate the effects of ROS increase on pancreatic cancer stem cell population and properties in this study. PANC-
1 cells were treated with different concentrations of H2O2 and the increase in ROS levels was investigated. Following
treatment with H2O2, the cancer stem cell population was analyzed by aldehyde dehydrogenase-positive (ALDH+) cell
population. Besides, the changes in cancer stem cell properties were determined by transwell migration, invasion assay,
and colony formation assay. According to our results, administration of 0.05 mM and 0.1 mM H2O2 to PANC-1 cells for
24 hours slightly increased ROS levels and decreased the cancer stem cell population. The decrease in cancer stem cell
population was significant at 0.1 mM H2O2 treated PANC-1 cells. Consistent with a decrease in the stem cell population,
the invasive and motility abilities were reduced and the number of colonies in soft agar was less when compared to control
groups. According to our results, slightly increased level of ROS suppresses the population of cancer stem cells (CSC)
and aggressiveness of pancreas adenocarcinoma cells.
Keywords: Cancer stem cell, pancreatic adenocarcinoma, reactive oxygen species, tumor aggressiveness.