Research Information System
XXIV th North American Testis Workshop, Florida, United States Of America, 19 April - 22 May 2017, pp.52, (Full Text)
Fertilin-β, Calmegin, Izumo-1, P34H, ACE and Fibronectin proteins on the surface of ram spermatozoa: Determined not only with the quantity but also with their distribution
Spermatozoas at developing stages obtained from testis and 3 different regions of epididymis. Determination of existence and localisation of Fertilin-β, Calmegin, Izumo-1, P34H, ACE and Fibronectin were analyzed quantatively via their protein expression profiles by western blotting technique and indirect immunofluorescence technique. Localisation changes of ram spermatozoa during development and maturation have been determined and also ejaculate and structural features of freezed-thawed ram spermatozoas with and without in vitro capacitation/acrosome reaction also been evaluated.
Fertilin-β, Calmegin, P34H proteins in caput, corpus, cauda and mature spermatozoas showed marking in different density and distrubition with. Freezed-thawed samples had lower density and marking than both ejaculate and cauda samples.
Marking was not obtained except Izumo-1 protein from the samples undergo in vitro capatitation/acrosome reaction. Marking of Izumo-1 protein was seen as increasing band formation through equatorial region on acrosome, after in vitro capacitation, however after acrosome reaction, the band formation was only equatorial region. In contrast to expected marking on spermatozoa head, non specific marking was obtained on different localization changing with the region in fibronectin antibody and samples. ACE antibody did not mark the samples. Region specific differences of proteins at kDa level were obtained with western blotting and possible isoforms specific to ram spermatozoa or proteins with similar epitops were marked.
The present work was supported by the Research Fund of Istanbul University.
Project No. 47033