Akademik Veri Yönetim Sistemi
V. International Enzyme and Bioprocess Days , İzmir, Türkiye, 27 - 29 Ağustos 2024, ss.34-36, (Özet Bildiri)
This study sought to identify and computationally analyze xylanases from rumen metagenome of Anatolian water buffalo. Thus, the rumen metagenome of Anatolian water buffaloes was sequenced using the Illumina NextSeq 550 next-generation sequencing platform, followed by bioinformatic analysis to assess microbial diversity and identify complete xylanase sequences. Next, the biophysicochemical properties of the xylanase sequences were analyzed using ProtParam, while their phylogenetic relationships were determined using the MEGA11 software. Multiple sequence alignment and analysis of B’-factor distribution were conducted to identify conserved residues and rigid regions, respectively, in the probable thermostable xylanases. The study investigated protein-ligand interactions through molecular docking analysis. The study found Firmicutes to be the most prevalent phylum, followed closely by Bacteroidetes. Several bacterial genera, including Prevotella, Methanobrevibacter, Butyrivibrio, Sarcina, Ruminococcus, Fibrobacter, Succiniclasticum, and Clostridium, were notably abundant. Biophysicochemical analysis identified four xylanases (AWBRMetXyn5, AWBRMetXyn10, AWBRMetXyn19) with favorable properties: aliphatic index > 80, instability index < 40, and melting temperature (Tm) > 65°C. Phylogenetic analysis grouped the xylanases into five families, mainly GH10. BANΔIT analysis showed three potential thermostable xylanases with significantly lower B'- factor values in the α1-β1 loop/short helix region compared to the reference enzyme XynAS9. Alignment analysis revealed conservation of eight key residues crucial for ligand interactions in three potential thermostable xylanases. Docking results suggested a significant role for the α2-β2 short helix, α6-β6 loop, α6, and α7-β7 short helix in AWBRMetXyn5, AWBRMetXyn10, and AWBRMetXyn19. Therefore, AWBRMetXyn5, AWBRMetXyn10, and AWBRMetXyn19 could be regarded as promising candidates for diverse industrial applications.