Evidence for oxidative stress in the hepatic mitochondria of bile duct ligated rats


Alptekin N., Mehmetcik G., Uysal M., AykacToker G.

PHARMACOLOGICAL RESEARCH, vol.36, no.3, pp.243-247, 1997 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 3
  • Publication Date: 1997
  • Doi Number: 10.1006/phrs.1997.0225
  • Title of Journal : PHARMACOLOGICAL RESEARCH
  • Page Numbers: pp.243-247

Abstract

Lipid peroxidation increased both in the liver homogenates and in the hepatic mitochondrial fraction of bile duct ligated (BDL)-rats. Although mitochondrial superoxide dismutase (SOD) activity did not change in the liver, glutathione (GSH) levels and glutathione peroxidase (GSH-Px) activity decreased in hepatic mitochondrial fraction of BDL-rats as compared to sham-operated rats. In addition, GSH-Px and glutathione transferase (GST) activities decreased but SOD activity remained unchanged in the post-mitochondrial fraction of the liver from BDL-rats. However, erythrocyte lipid peroxide and GSH levels did not change in BDL-rats. In conclusion, our results show that the disturbance of oxidant-antioxidant balance especially in mitochondria may be responsible for cholestatic injury in BDL-rats. (C) 1997 The Italian Pharmacological Society.