In this study, 94 clinically ill 11-28-day-old chicks belonging to eight broiler units from the Marmara region were investigated clinically for changes in hematocrit values and for the presence of chicken anemia virus (CAV) DNA. CAV DNA was detected by polymerase chain reaction in the thymus of 8.5% of the chicks. These chicks showed clinical signs of diarrhea, anorexia, depression, and growth retardation. The hematocrit values of these chicks were between 24% and 38%. At necropsy, hemorrhages were observed in the leg and pectoral muscles. Atrophy was noted in the thymus and in the bursa of Fabricius of positive chicks, and hemorrhages in the proventriculus of one positive chick were observed. This report describes the first detection of CAV DNA in chicks in Turkey.