Retinoic acid modulates peripheral blood helper innate lymphoid cell composition in vitro in patients with multiple sclerosis

Özkoşar A., ÖKTELİK F. B., GELMEZ M. Y., Öztürk Erden S., GÜNDÜZ T., KÜRTÜNCÜ M., ...More

Journal of Neuroimmunology, vol.398, 2025 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 398
  • Publication Date: 2025
  • Doi Number: 10.1016/j.jneuroim.2024.578489
  • Journal Name: Journal of Neuroimmunology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Psycinfo, Veterinary Science Database
  • Keywords: Innate lymphoid cell, Innate lymphoid cell plasticity, Interleukin-10, Multiple sclerosis, Retinoic acid
  • Istanbul University Affiliated: Yes

Abstract

This study investigates the frequency and numbers of circulating helper innate lymphoid cells (ILCs) in untreated relapsing-remitting multiple sclerosis (RRMS) patients, focusing on intracellular IL-10 and CCR6 expressions under IL-2, IL-33, and retinoic acid (RA) stimulation in vitro and their associations with clinical features in RRMS. In RRMS patients, ILC1 levels were notably higher upon IL-2 + IL-33 + RA stimulation, while ILC2 levels, particularly the c-Kit+ ILC2 and CCR6+ ILC2 subsets, were significantly lower compared to unstimulated conditions. Additionally, IL-10+ ILC1 levels were elevated. The ratios of IL-10+ ILC1/ILC1, c-Kit+ ILC2/c-Kit− ILC2, and CCR6+ ILC2/ILC2 were associated with the progression index (PI) in RRMS patients.