THE PROTECTIVE EFFECTS OF TETRANDRINE AGAINST TO HISTOLOGICAL, SPERMATOLOGICAL AND OXIDATIVE DAMAGE INDUCED BY AROCLOR 1254 ON THE MALE RATS REPRODUCTIVE SYSTEM


özdemir f., TAŞDEMİR A., ÇİFTÇİ O., AYDIN M., TÜRKMEN N. B.

Sabiad , vol.6, no.1, pp.80-87, 2023 (Peer-Reviewed Journal) identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 1
  • Publication Date: 2023
  • Doi Number: 10.26650/jarhs2023-1181839
  • Journal Name: Sabiad
  • Journal Indexes: TR DİZİN (ULAKBİM)
  • Page Numbers: pp.80-87
  • Istanbul University Affiliated: Yes

Abstract

Objectives: Aroclor (AR) 1254; has many adverse effects on male reproduction such as carcinogenic, teratogenic, immune and endocrine disruption problems. Tetrandrine (TET), a bisbenzillisoquinoline alkaloid isolated from the root of Stephania tetrandra S. Moore, has protective effects such as immunomodulatory, anti-cancer, and anti-inflammatory. The objective of this study was to investigate the possible curative effects of TET therapy against testicular damage (histological, spermatological and oxidative damage) induced by AR1254. Materials and Methods: Twenty-eight male rats were randomly divided into four equal-sized groups: a control group; (1 ml of corn oil by gastric oral gavage), AR1254 group; (2 mg/kg) AR1254 administered intraperitoneally), TET group; (TET by gastric oral gavage 30 mg/kg) and AR 1254 + TET group;(Aroclor 1254 and TET administered together at the same doses as the previous groups. Results: The AR1254 treatment caused morphological and spermatological damage on testis tissue; oedema vacuolization and congestion, in interstitial area, reduction in spermatogenic cells, arrested spermatocytes at different stages of spermatogenesis, shedding of spermatogenic serial cells into tubular lumens, a decline in epididymal sperm concentrations, sperm motility and a rise in abnormal sperm ratios. The AR1254 administration induced an increase in the oxidative parameters and a decrease in enzymatic and nonenzymatic antioxidant levels. The TET treatment significantly ameliorated histological, oxidative, and sperm damage caused by AR1254. Conclusion: This study demonstrated the protective effects of TET against AR1254-induced male rat reproductive damage.