A High-Performance Liquid Chromatographic Method for the Determination of Meropenem in Serum.

Dincel D., Sagirli O., Topcu G.

Journal of chromatographic science, vol.58, pp.144-150, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 58
  • Publication Date: 2020
  • Doi Number: 10.1093/chromsci/bmz087
  • Journal Name: Journal of chromatographic science
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, Food Science & Technology Abstracts, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.144-150
  • Keywords: Carbapenems, Meropenem, High-Performance Liquid Chromatography, Human Serum, Validation, Therapeutic Drug Monitoring, BETA-LACTAM ANTIBIOTICS, HUMAN PLASMA, RAT PLASMA, METABOLISM, ASSAY
  • Istanbul University Affiliated: Yes


In this study, a new, sensitive and selective high-performance liquid chromatographic method was developed for the determination of meropenem (MEM) in human serum. In the developed

method, C18 column (3.9 × 150 mm, 5 μm) was selected as stationary phase at 30◦C, and methanol:acetic acid solution mixture was used as mobile phase with gradient program. Chromatographic separation was carried out at a flow rate of 1 mL/min, and detection was performed at 300 nm with diode array detector. Doripenem was selected as an internal standard, and the analytes were extracted from serum using protein precipitation method with ortho-phosphoric acid: methanol. Detection wavelength was selected as 300 nm. The developed method was validated according to International Council for Harmonisation (ICH) guidelines. The calibration curve was linear over a concentration range of 4–240 μg/mL with correlation coefficient of 0.9985. The limit of detection and limit of quantification values were found as 0.057 and 0.192 μg/mL, respectively. The validated method was successfully applied for the determination of MEM in human serum samples collected from patient volunteers at different time intervals, and therapeutic drug monitoring of MEM has been investigated.