Akademik Veri Yönetim Sistemi
9th international istanbul scientific research congress, İstanbul, Türkiye, 14 - 15 Mayıs 2022, ss.246
Curcumin is a valuable phenolic compound which has been used in Asian medicine for ages and has anticancer, antiinflamatory, antioxydant and hepatoprotective effects(1.2,3,4). A few studies have been carried out with curcumin in terms of in vitro oocyte maturation and embryonic development, but these have remained limited in number and species in large farm animals. The aim of our project is to investigate the basic effects of different doses of curcumin on maturation of sheep oocytes.(0, 0.1, 1, 10 mM) and to provide preliminary data for researchers to investigate its effects on in vitro culture of targeted sheep embryos in the future. This study will have a unique value in terms of investigating the effect of curcumin, which has positive and negative effects on the development of mouse and other large animal oocytes and embryos, in a previously untested species, in ovine oocytes.When compared to the control group in the polar group, 88.6% of the cells showed good growth at 0.1 µm dose, 11.4% showed poor growth, and no statistically significant improvement was observed in cells treated with 0.1 µM dose curcumin. However, it was observed that 60% of the cells cultured with 1 µM curcumin showed good growth and 40% of the cells showed poor growth compared to the control group. As a result, a statistically significant difference was observed in the group treated with 1 µm dose of curcumin. In the polar group, further significance was found in the statistics of oocytes incubated with 10 µm dose of curcumin, and 25.7% of the cells continued to develop at this dose, all groups (0, 0.1, 1 and 10 µM) were compared with each other, and no statistically different finding was found between the groups (p>0.05). Keywords: Curcumin,