According to most of the educators, scientific literacy means the understanding of concepts, theories, principles, processes of science, awareness of relationship, among science, technology and society. Currently, to bring up as Science and Technology literacy has become necessity in order to brace new generation to future. At this point, science and technology course has an important role in achieving this aim. The aim of this study is to improve valid and reliable test in order to determine readiness of 715 class students about learning "Structure of Atom" course at Science and Technology course. First of all, concepts and acquisitions which are basis for the subject were determined and acquisitions were classified according to revised Bloom Taxonomy. Then, literature review was done in order to determine misconceptions and learning difficulties of students and item pool including 33 multiple-choice items was created. After creating table of specifications, the test was presented to the evaluation of chemistry instructors and Science teachers for validity. According to the feedbacks, the test was reduced to 26 items for pilot study and applied on 336 students at 7th class. According to item analysis result, it was determined that discrimination index of 9 items was below 0.2. These items were excluded from the test and a readiness test of 17 items was prepared. Reliability coefficient of this test was found 0.78. (C) 2014 The Authors. Published by Elsevier Ltd.