New spectrofluorimetric method for the determination of nizatidine in bulk form and in pharmaceutical preparations


Karasakal A., Ulu S.

OPTICS AND SPECTROSCOPY, cilt.115, ss.306-309, 2013 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 115 Konu: 2
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1134/s0030400x13080031
  • Dergi Adı: OPTICS AND SPECTROSCOPY
  • Sayfa Sayıları: ss.306-309

Özet

A simple, accurate and highly sensitive spectrofluorimetric method has been developed for determination of nizatidine in pure form and in pharmaceutical dosage forms. The method is based on the reaction between nizatidine and 1-dimethylaminonaphthalene-5-sulphonyl chloride in carbonate buffer, pH 10.5, to yield a highly fluorescent derivative peaking at 513 nm after excitation at 367 nm. Various factors affecting the fluorescence intensity of nizatidin-dansyl derivative were studied and conditions were optimized. The method was validated as per ICH guidelines. The fluorescence concentration plot was rectilinear over the range of 25-300 ng/mL. Limit of detection and limit of quantification were calculated as 11.71 and 35.73 ng/mL, respectively. The proposed method was successfully applied to pharmaceutical preparations.