Development and validation of a sensitive spectrofluorimetric method for the determination of amoxapine in human plasma and urine


Karasakal A., Ulu S. T.

LUMINESCENCE, vol.29, no.3, pp.284-287, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 3
  • Publication Date: 2014
  • Doi Number: 10.1002/bio.2541
  • Journal Name: LUMINESCENCE
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.284-287

Abstract

A selective and sensitive spectrofluorimetric method was developed and validated for the determination of amoxapine in human plasma and urine. The developed method is based on labeling with 5-dimethylaminonaphthalene-1-sulfonyl chloride (dansyl chloride) and monitoring at 397nm (excitation)/514nm (emission). The method was validated for linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, recovery and robustness. The calibration curves were linear over a concentration range of 250-2500 and 50-1250ng/mL for plasma and urine, respectively. The LOD values were calculated to be 13.31 and 13.17ng/mL for plasma and urine, respectively. The proposed method was applied to study of amoxapine in human plasma and urine. Copyright (c) 2013 John Wiley & Sons, Ltd.