A New Spectrophotometric Method for the Determination of Tianeptine in Tablets Using Ion-Pair Reagents

Ulu S., Aydogmus Z.

CHEMICAL & PHARMACEUTICAL BULLETIN, vol.56, no.12, pp.1635-1638, 2008 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 56 Issue: 12
  • Publication Date: 2008
  • Doi Number: 10.1248/cpb.56.1635
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1635-1638
  • Keywords: tianeptine, spectrophotometry, ion pair, pharmaceutical preparation, validation, PERFORMANCE LIQUID-CHROMATOGRAPHY, FLUORESCENCE
  • Istanbul University Affiliated: Yes


A new rapid and sensitive procedure assay is proposed for the spectrophotometric determination of tianeptine. The developed method involves formation of colored chloroform extractable ion-pair complexes of tianeptine with bromophenol blue (BPB), bromocresol green (BCG), bromothymol blue (BTB) and methyl orange (MO) in acidic medium. Beer's law is obeyed in the concentration ranges 3.0-12.0, 4.0-16.0, 4.0-14.0 and 2.0-10.0 mu g ml(-1) with BPB, BCG, BTB and MO, respectively. The detection limit of tianeptine was found to be 1.8.mu g ml(-1) for BPB, 2.0 for BCG, 2.0 mu g ml(-1) for BTB and 1.0 mu g ml(-1) for MO. Validation of the method was performed in terms of linearity, limit of detection (LOD), quantification (LOQ), accuracy and precision. Common excipients used as additives in pharmaceutical preparations do not interfere in the proposed method. The proposed method has been applied to determination of the examined drugs in pharmaceutical formulations and the results demonstrated that the method is equally accurate, precise, and reproducible as the official method. The t-test showed no significant difference at 95% confidence level.