In this study, effects of DGAT1 K232 polymorphism and the allele frequency differences of "K" (alanine variant) and "L" (lysine variant) on some cattle milk qualities and some microbiological parameters which pose a risk on consumer health were investigated. For this purpose, 2 years old 50 cattles were used as material (25 Holstein race and 25 Native Black race). Blood samples were collected for determining the allele frequencies of DGAT1 gene. Then, during the experimental period (1 month) the milk of the cattles were collected once a week and the milk samples has been explored for some important foodborne pathogens (Total Aerobic Bacteria, Coliforms, Escherichia coli, Listeria monocytogenes and Staphylococcus aureus) and milk quality parameters (pH, fat, density and acidity). According to the findings, it has been identified that DGAT1 gene frequency differences were significantly effective on some the parameters of milk qualities and the growth of coliforms, Esherichia coli and Staphylococcus aureus.