Evaluating Enzymatic Productivity-The Missing Link to Enzyme Utility

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Siddiqui K. S., Ertan H., Poljak A., Bridge W. J.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, cilt.23, sa.13, 2022 (SCI-Expanded)

• Yayın Türü: Makale / Derleme
• Cilt numarası: 23 Sayı: 13
• Basım Tarihi: 2022
• Doi Numarası: 10.3390/ijms23136908
• Dergi Adı: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
• Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
• Anahtar Kelimeler: industrial biotechnology, enzyme, productivity, stability, kinetics, inhibition, biocatalysis, immobilized, chemical modification, genetic modification, ISOTHERMAL TITRATION CALORIMETRY, TEMPERATURE, DELIGNIFICATION, IMMOBILIZATION, INHIBITION, HYDROLYSIS
• Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
• İstanbul Üniversitesi Adresli: Hayır

Özet

Kinetic productivity analysis is critical to the characterization of enzyme catalytic performance and capacity. However, productivity analysis has been largely overlooked in the published literature. Less than 0.01% of studies which report on enzyme characterization present productivity analysis, despite the fact that this is the only measurement method that provides a reliable indicator of potential commercial utility. Here, we argue that reporting productivity data involving native, modified, and immobilized enzymes under different reaction conditions will be of immense value in optimizing enzymatic processes, with a view to accelerating biotechnological applications. With the use of examples from wide-ranging studies, we demonstrate that productivity is a measure of critical importance to the translational and commercial use of enzymes and processes that employ them. We conclude the review by suggesting steps to maximize the productivity of enzyme catalyzed reactions.