Akademik Veri Yönetim Sistemi
Naunyn-Schmiedeberg's Archives of Pharmacology , cilt.397, sa.7, ss.1-11, 2024 (SCI-Expanded)
Melatonin is a powerful endogenous antioxidant hormone. Its healing efects on energy balance and neuronal damage
associated with oxidative metabolism disorders have been reported in pathologic conditions. We aimed to determinate the
utility of melatonin on neuronal damage, synaptic transmission, and energy balance in the brain tissue of rats with sepsis
induced with LPS. Rats was divided into four groups such as control, LPS (20 mg/kg i.p.), melatonin (10 mg/kg i.p. ×3),
and LPS+Melatonin (LPS+Mel). After 6 h from the frst injection, rats were decapitated, and also tissue and serum samples were taken. Lipid peroxidation and neuron-specifc enolase (NSE) levels were determined from the serum in all group.
High energy compounds, creatine, and creatine phosphate are measured by HPLC methods from the homogenized tissue.
Counts of living neurons are marked with NeuN (neuronal nuclei), degenerated neurons are marked with S100-ß and synaptic
vesicles transmission is analyzed with synaptophysin antibodies immunoreactivities. One-way ANOVA and post hoc Tukey
tests were used to statistical analysis. In LPS group, AMP, ATP, creatine, and creatine phosphate levels were signifcantly
decreased (p<0.05), and also ADP levels were signifcantly increased compared with the other groups (p<0.01). Living
neurons counts were signifcantly decreased in LPS (p<0.01), melatonin, and LPS+Melatonin (p<0.05) groups compared
with control. Degenerated neurons counts were increased in LPS group compared with control (p<0.01) and also decreased
in both of melatonin and LPS+Melatonin groups (p<0.01). Synaptophysin immunoreactivity was decreased in LPS group
compared with the other groups (p<0.05). We observed that melatonin administration prevents neuronal damage, regulates
energy metabolism, and protects synaptic vesicle proteins from sepsis-induced reduction