REAL-TIME POLYMERASE CHAIN REACTION VERSUS CONVENTIONAL PCR: A COMPARISON BETWEEN TWO METHODS FOR THE DETECTION OF FUSOBACTERIUM NUCLEATUM IN SALIVA, NASOPHARYNGEAL SECRETION AND MIDDLE EAR EFFUSION SAMPLES

Topcuoglu N., Paltura C., Külekçi M., Ustek D., Kulekci G.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.27, sa.3, ss.3825-3828, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 3
  • Basım Tarihi: 2013
  • Doi Numarası: 10.5504/bbeq.2013.0022
  • Dergi Adı: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3825-3828
  • Anahtar Kelimeler: middle ear effusion, saliva, nasopharyngeal secretion, Fusobacterium nucleatum, PCR, real-time PCR, biofilm, ACUTE OTITIS-MEDIA, PORPHYROMONAS-GINGIVALIS, BACTERIAL PATHOGENS, PERIODONTAL STATUS, CHILDREN, MICROBIOTA, CULTURE
  • İstanbul Üniversitesi Adresli: Evet

Özet

Oral and middle ear infections are biofilm related infections. Fusobacterium nucleatum is considered to be a key species in dental biofilm formation. The aim of this study was to compare conventional and real-time PCR, for the detection of F. nucleatum in saliva, nasopharyngeal secretion and middle ear effusion samples. Sixty samples were collected from 20 patients with otitis media with effusion. Species-specific 165 rRNA primers were used for conventional PCR and species-specific primers and TaqMan probes were used for real-time PCR analysis. Real-time PCR had a better performance than conventional PCR in detecting bacteria in the analyzed samples. The tested real-time PCR assay can identify) and identify) F nucleatum in biofilm samples with a high degree of sensitivity when compared with conventional PCR techniques.