Patients with epileptic seizures in the intensive care unit is distinguished from other diseases by IL-1beta and IL-8 levels but not markers of glial activation.

Şanlı E., Altundağ E., İlgen Uslu F., Karaaslan Z., Akbayır E., Koral G., ...Daha Fazla

XVII Workshop on Neurobiology of Epilepsy – WONOEP 2023, Dublin, İrlanda, 28 - 31 Ağustos 2023, ss.24

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Dublin
  • Basıldığı Ülke: İrlanda
  • Sayfa Sayıları: ss.24
  • İstanbul Üniversitesi Adresli: Evet

Özet

Objective: Chronic neuroinflammation has been implicated in the physiopathology of epilepsy. Our aim was to investigate markers of inflammation and glial activation in sera and peripheral blood of intensive care unit (ICU) patients with epilepsy with different etiologies.

Method: The study included 9 Epilepsia partialis continua (EPC) patients, patients with other type of epilepsy (n=14), 16 ICU patients without seizures and 10 healthy donors. Levels of pro-inflammatory cytokines, complement factors and glial activation markers were measured by ELISA and magnetic bead-based assay (Luminex). Peripheral blood expressions of NF-κB, CASP1, NLRP3, IL-1β, TNFRSF1A, IL-18, and IL-18R1 genes were determined by Real-Time Polymerase Chain Reaction (qPCR).

Conclusion: Levels of all markers of inflammation except C3a were higher in sera of epilepsy and other ICU patients compared to healthy controls. Epilepsy patients showed significantly lower serum IL-1beta and IL-8 levels than non-epilepsy ICU patients, whereas both groups showed comparable levels of C3a, C5a, TNF-alpha, IL-6, UCHL1, GFAP, TREM2 and HMGB1. Epilepsy patients showed significantly higher IL-8 levels than patients with EPC. There were no differences among groups in terms of NF-κB and inflammasome factor gene expression levels.

Discussion: Epilepsy in ICU is distinguished by reduced IL-1beta and IL-8 levels (but not markers of glial activation) from other ICU patients and thus these two markers might be utilized as biomarkers. IL-8 is a suppressor of inflammatory M1 microglia, and reduced IL-8 levels may be associated with increased glial activation and worse prognosis in EPC.