Akademik Veri Yönetim Sistemi
Journal of Food Composition and Analysis, cilt.148, 2025 (SCI-Expanded)
The effects of tebuconazole, carbendazim, and fludioxonil on the production of deoxynivalenol, 3-acetyl deoxynivalenol, and 15-acetyl deoxynivalenol in F. graminearum and F. culmorum were investigated. The mycotoxin production capacities of the two Fusarium species were determined to be 15-acetyl deoxynivalenol and 3-acetyl deoxynivalenol by amplifying the 586 bp and 864 bp deoxyribonucleic acid fragments by a polymerase chain reaction (PCR), respectively. A sensitive and reliable a HPLC-PDA method was developed and validated to quantify these mycotoxins. The mobile phase, flow rate, and column temperature were optimized. The limits of detection and quantification were found to be in the range of 1.22–9.35 ng/mL and 4.06–31.16 ng/mL, respectively. This method quantified these mycotoxins with high sensitivity in references and isolates exposed to tebuconazole, carbendazim, and fludioxonil. These compounds affected mycotoxin production at varying levels, with deoxynivalenol being the most produced mycotoxin in both Fusarium species. The findings indicated that a PCR could be used to detect mycotoxin production capacity. Since B-trichothecenes transmitted to humans and animals can cause serious health problems, it is important to incorporate this validated methodology into routine laboratory analysis to prevent their adverse effects on public health.