Potential therapeutic effects of ethyl pyruvate in an experimental rat appendicitis model


Sag S., Elemen L., Masrabaci K., Recber S. F., Sonmez Y., Aydin S., ...More

JOURNAL OF PEDIATRIC SURGERY, vol.57, no.10, pp.457-462, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 10
  • Publication Date: 2022
  • Doi Number: 10.1016/j.jpedsurg.2021.11.016
  • Journal Name: JOURNAL OF PEDIATRIC SURGERY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.457-462
  • Keywords: Appendicitis, Child, Conservative treatment, Rats, ACUTE UNCOMPLICATED APPENDICITIS, CHILDREN, INTERLEUKIN-6, MARKERS, INJURY
  • Istanbul University Affiliated: Yes

Abstract

Background: Pathophysiology of appendicitis is associated with the underlying inflammatory processes. Ethyl pyruvate (EP) has potent antioxidant and anti inflammatory properties. In this study, we aimed to investigate the effects of EP on the treatment of appendicitis and to examine whether adding EP to the antibiotic treatment could increases the effectiveness of the treatment in a rat appendicitis model. Method: Thirty two Wistar rats, which had previously created appendicitis, were randomly divided into 4 groups: Group 1 (0.1 ml saline solution), Group 2 (15 mg/kg ceftriaxone), Group 3 (50 mg/kg EP), Group 4 (EP 50 mg/kg + ceftriaxone 15 mg/kg). In all groups, saline solution, ceftriaxone and EP were adminis-tered intraperitoneally and the same procedure was repeated twice a day for the following five days. On day 6, the rats underwent relaparotomy and then intraabdominal findings were recorded. Histopatholog-ical examination and interleukin 6 (IL 6) level were performed on appendiceal specimens. Results: Intra abdominal adhesion score was significantly lower in Group 4 than in Group 1. Total inflam-mation score was significantly lower in Group 2 than in Group 1 and was significantly lower in Group 4 than in Group 3 and 1. IL 6 level was significantly lower in Group 4 than in Group 3 and 1. Conclusion: We found that adding EP to the antibiotic therapy increased the efficacy of the treatment in the rat appendicitis model. Further studies are required to apply our findings to the clinical setting. (c) 2021 Elsevier Inc. All rights reserved.