Biospectroscopy screening and molecular fingerprinting of gastric cancer cases from biofluids by vibrational spectroscopy allied with chemometrics

Tugrul F., Akin Geyik G., Peker Cengiz B., Yalinbaş Kaya B., KARUK ELMAS Ş. N., Yilmaz I., ...More

Scientific Reports, vol.15, no.1, 2025 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 15 Issue: 1
  • Publication Date: 2025
  • Doi Number: 10.1038/s41598-025-09912-0
  • Journal Name: Scientific Reports
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Chemical Abstracts Core, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
  • Keywords: Cancer, Chemometrics, Gastric, Infrared spectroscopy
  • Istanbul University Affiliated: No

Abstract

The present study aims to develop a new molecular diagnostic modality based upon mid–infrared (mid–IR) spectroscopy allied with chemometrics for the diagnosis of gastric cancer (GC) from diverse biofluids. The characterization over the spectral data specified the changes at molecular level caused from the synthesis of main biomolecules like lipids, proteins and carbohydrates was performed. The spectral data from the cancer and their control cases were analyzed by practicing the supervised and unsupervised chemometrics analysis including PCA, SIMCA, LDA and HCA. The results indicated that remarkable changes were occurred in the content of biomolecules for GC cases due to energy necessities. 100% of the cancer cases from blood serum, blood plasma and saliva biofluids were successfully discriminated from their control specimens by LDA. Consequently, this research established the potential of mid–IR spectroscopy allied with chemometrics for evaluating biochemical changes of GC cases with high sensitivity and precision.