Akademik Veri Yönetim Sistemi
38th EFI Conference Immunogenetics , Praha, Çek Cumhuriyeti, 14 - 17 Mayıs 2025, ss.46-47, (Özet Bildiri)
Chimerism monitoring after HSCT is critical for assessing graftsuccess and detecting potential transplantation complications atan early stage. Conventional methods such as STR analysis andqPCR have limitations in terms of sensitivity. NGS has emergedas a groundbreaking approach, offering exceptional sensitivityand accuracy in detecting donor and recipient cell populations.This study aims to compare chimerism analysis using NGSwith the STR method and evaluate its sensitivity. This study in-cluded 34 samples: 14 from HSCT patients at Istanbul MedicalFaculty Haematology Department and 10 from EQCs. Analyseswere conducted at the Istanbul Faculty of Medicine TissueTyping Laboratory. The samples included DNA extracted fromperipheral blood, bone marrow, intra-abdominal ascitic fluid,and liver biopsy material. DNA isolation was performed usingthe Promega Maxwell RSC kit, and the samples were analysedusing both STR and NGS methods. STR analysis was conductedusing the Qiagen IDPlex Plus kit with fragment analysis, while NGS analysis was performed using the One Lambda Devyser™Chimerism NGS Assay kit. Of the 34 samples analysed by STR,16 were reported as full chimerism. However, NGS analysis de-tected microchimerism or mixed chimerism in 14 of these sam-ples. Additionally, percentage differences were observed in thesamples identified as mixed chimerism. NGS-based chimerismanalysis demonstrates significantly higher sensitivity comparedto conventional STR methods and enables the early detectionof minimal residual disease. In several samples classified as“complete chimerism” by STR, NGS identified the presence ofmicrochimerism, suggesting that NGS provides a more reliablemethod for predicting early graft failure and relapse risk. Theintegration of NGS into clinical practice may mark a new era inpost-transplant patient management, offering improved moni-toring and early intervention strategies.