Dissection of Anoplophora glabripennis (Coleoptera: Cerambycidae) larval tissues for physiological and molecular studies

Torson A. S., Des Marteaux L. E., Bowman S., Zhang M. L., Ong K., Doucet D., ...More

CANADIAN ENTOMOLOGIST, vol.152, no.3, pp.399-409, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 152 Issue: 3
  • Publication Date: 2020
  • Doi Number: 10.4039/tce.2020.22
  • Journal Name: CANADIAN ENTOMOLOGIST
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Agricultural & Environmental Science Database, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, CAB Abstracts, Geobase, Veterinary Science Database
  • Page Numbers: pp.399-409
  • Istanbul University Affiliated: No

Abstract

Many biological processes are partitioned among organs and tissues, necessitating tissue-specific or organ-specific analysis (particularly for comparative -omics studies). Standardised techniques for tissue identification and dissection are therefore imperative for comparing among studies. Here we describe dissection protocols for isolating six key tissues/organs from larvae of the Asian longhorned beetle, Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae): the supraoesophageal ganglion, posterior midgut, hindgut, Malpighian tubules, fat body, and thoracic muscle. We also describe how to extract haemolymph and preserve whole larvae for measurements such as protein, lipid, and carbohydrate content. We include dissection protocols for both fresh-killed and previously frozen specimens. Although this protocol is developed for A. glabripennis, it should allow standardised tissue collection from larvae of other cerambycids and be readily transferrable to other beetle taxa with similar larval morphology.