CROSS-REACTIVITY OF OVINE IGE WITH ANTI-HUMAN IGE


YILMAZ H. , MORGAN K., ROE J.

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, vol.104, no.3, pp.277-280, 1994 (Journal Indexed in SCI) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 104 Issue: 3
  • Publication Date: 1994
  • Doi Number: 10.1159/000236677
  • Journal Name: INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.277-280

Abstract

Cross-reactivity between ovine and human IgE was investigated using monoclonal antibodies to human IgE heavy chain. Western immunoblotting, reverse cutaneous anaphylaxis (RCA), passive cutaneous anaphylaxis inhibition (PCAI) and reverse passive cutaneous anaphylaxis (RPCA) tests were all used to assess cross-reactivity between the monoclonals and ovine IgE. No cross-reactivity was demonstrated using Western immunoblotting, RCA and PCAI tests. However, evidence for homology in the region of the molecule concerned with mast cell binding was demonstrated by RPCA performed in sheep skin primed with human myeloma IgE.