Mıcrobıal bıofılms on contact lenses and theır susceptıbılıty to multıpurpose solutıons.
5th INTERNATIONAL PARIS CONGRESS ON MEDICAL & HEALTH SCIENCES , Paris, Fransa, 19 - 21 Haziran 2026, ss.199, (Tam Metin Bildiri)
- Yayın Türü: Bildiri / Tam Metin Bildiri
- Basıldığı Şehir: Paris
- Basıldığı Ülke: Fransa
- Sayfa Sayıları: ss.199
- İstanbul Üniversitesi Adresli: Evet
Özet
Background: Contact lens (CL) wear is widely used for refractive corrections and cosmetic purposes, while it also remains a major predisposing factor for microbial keratitis. Among bacterial pathogens, Pseudomonas aeruginosa and Staphylococcus aureus are the most common agents of CL-associated infections due to their strong biofilm-forming abilities. Since studies evaluating the antibiofilm activities of multipurpose solutions (MPSs) are very limited, biofilms were established on different CL types, and antibiofilm activities of commercially available MPSs were evaluated in this study. Materials and Methods: Seven MPSs’ biofilm inhibition and mature biofilm eradication activities were evaluated against P. aeruginosa ATCC 27853 and S. aureus ATCC 29213 on different CL types using viable cell counts, OD measurements, and crystal violet staining methods. Results: According to biofilm formation experiments, the highest biofilm was observed on used/worn CLs, and denser biofilm masses were generally detected on low-water-content CLs and also on expired CLs. In biofilm inhibition assays, the most active MPS against P. aeruginosa was Acuvue Revitalens, providing up to 50-60% inhibition, while against S. aureus, Elegance Aqua Fresh and ReNu Advanced were more effective. On mature biofilms, ≥3-log10 cfu/mL bactericidal activity against P. aeruginosa was achieved by Acuvue Revitalens; against S. aureus, bactericidal activity was achieved by almost all MPSs except TTO and Oculid. Discussion and Conclusion: Antibiofilm activities of MPSs are strongly dependent on the stage of biofilm development and further modulated by CLs’ properties. S. aureus biofilms were more susceptible to the tested MPSs than P. aeruginosa, consistent with the higher intrinsic resistance of Gram-negative biofilms. Key Words: Contact lenses; Multipurpose solutions; Biofilm inhibition; Biofilm eradication; Pseudomonas aeruginosa; Staphylococcus aureus