Mg/Al-NO3 Layered Double Hydroxide (LDH) As Nonviral Gene Carrier Nanoparticular System
5th International Group Congress – “Vet-Istanbul Group” & 8th International Scientific Meeting “Days of Veterinary Medicine-2018, Ohrid, Macedonia, 23 - 27 September 2018, vol.1, no.1, pp.128, (Full Text)
- Publication Type: Conference Paper / Full Text
- Volume: 1
- City: Ohrid
- Country: Macedonia
- Page Numbers: pp.128
- Istanbul University Affiliated: Yes
Abstract
Layered Double Hydroxide (LDH) is a hydrotalcite-like, anionic clay having the general formula [MIInMIII(OH)2+2n]+ (Am−)1/m·H2O. In this formula MII, MIII and Am- represent divalent a metal cation (e.g. Mg2+, Zn2+, Cu2+ etc), a trivalent metal cation (e.g. Al3+, Fe3+, Ga3+ etc) and an anionic type (e.g. Cl-, NO3-,CO32- etc), respectively. In recent years, the applications of LDH, which was called as host-guest materials, in the biomedical area have become important. Especially, its properties such as good biocompatibility, low cytotoxicity, high loading capacity, diverse functionality, adjustable particle size, targeted delivery and pH controlled release of guest molecules, protection biomolecules from external chemical and biological attack make LDH an effective non-viral vector for gene therapy. The key objective of this study is to investigate the effects of the synthesis parameters on the properties of the LDH as a non-viral gene carrier nanoparticular system and to evalute its transfection efficacy. For this purpose Mg/Al-NO3 LDH was prepared by coprecipitation method then hydrothermal treatment was carried out in autoclave. The effects of the reaction conditions such as temperature, time and stirring rate on the particle size were investigated. Experiments were carried out at 80 and 100 °C, in range of 2-48 hours and with 250, 1000, 1500 rpm, respectively. As the result of these studies, the optimum particle size of 74,52 nm, the polydispersity index (PDI) of 0,124 and the zeta potential (mV) of 52,8 were obtained at 80 °C, 48 h and 250 rpm. The preliminary in vitro studies were carried out by using Human Embryonic Kidney (HEK293T) cell line and the degree of transfection efficiency was measured as 24% by flow cytometry. It was concluded that nano-sized Mg/Al-NO3 LDH synthesized in this study can be used as a potential nontoxic nonviral gene carrier system.
Acknowledgements: This study was funded by Istanbul University with the project number: BYP-2017-23682 and TSG-2017-20659 and by TÜBİTAK with project number 117O345