The effect of miR30c-2-3p on METTL14 and its role in migration inhibition in breast cancer

ESHG 2025, Milan, İtalya, 24 - 27 Mayıs 2025, (Yayınlanmadı)

Abstract:

Background: High expression of miR30c-2-3p is linked to good prognosis in breast cancer (BC). Analysis using the GEPIA2

database revealed increased METTL14(methyltransferases) expression in BC compared to healthy tissues. Considering the

regulatory e"ects of miRNAs on m6A modifications, this study aimed to investigate miR30c-2-3p’s role in regulating m6A

modifications, its impact on migration, and its relationship with BC. Material and Methods: MCF12A (healthy) and MCF7 (BC)

cells were transfected with miR30c-2-3p mimics. miR30c-2-3p and METTL14 RNA levels were measured by RT-PCR, while

protein levels were assessed via western blot (WB). Migration was evaluated using scratch assays, and m6A levels were

determined through a colorimetric method. Results: Transfection with miRNA mimics significantly increased miR30c-2-3p

expression in MCF7 and MCF12A cells (p<0.01). In MCF7 cells, METTL14 expression decreased by 39.4%, 50.9% at the RNA level

and 79%, 56.2% at the protein level after 24 and 48 hours, respectively (p<0.001). m6A levels reduced by 28.9%, 46.2%, and

migration decreased by 30%, 44%, respectively. In MCF12A cells, METTL14 expression decreased by 25.6%, 30.9% at the RNA

level and 40.9%, 43.2% at the protein level at 24 and 48 hours, respectively (p<0.001). m6A levels reduced by 83.7%, 63.9%, and

migration inhibited by 66%, 72%, respectively. Conclusion: miR30c-2-3p overexpression reduced METTL14 and m6A levels

while inhibiting migration. Although migration results decreased in both cell lines, MCF7 demonstrated less migration compared

to MCF12A, likely due to the activation of alternative pathways. These findings highlight miR30c-2-3p’s role in BC biology and

its potential as a therapeutic target.