Periodontitis and peri-implantitis tissue levels of Treponema denticola-CTLP and its MMP-8 activating ability

Petain S., KASNAK G., FIRATLI H. E., Tervahartiala T., Gürsoy U. K., Sorsa T.

Acta Histochemica, cilt.123, sa.6, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 123 Sayı: 6
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.acthis.2021.151767
  • Dergi Adı: Acta Histochemica
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Arts and Humanities Citation Index (AHCI), Scopus, Academic Search Premier, Aerospace Database, Communication Abstracts, EMBASE, MEDLINE, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: MMP-8, collagenase-2, Td-CTLP, Treponema denticola, Periodontitis, Peri-implantitis, CONSENSUS REPORT, BIOMARKERS, PROTEINASE, EXPRESSION, DISEASES, CELLS
  • İstanbul Üniversitesi Adresli: Evet

Özet

© 2021 The AuthorsBackground and Aims: Chymotrypsin-like-proteinase of Treponema denticola (Td-CTLP) can stimulate the protein expression and activation of matrix metalloproteinase (MMP)-8 (or collagenase-2), a potent tissue destructive enzyme from gingival cells in vitro. The aims of this study were 1) to demonstrate the proMMP-8 (or latent MMP-8) activation by Td-CTLP in vitro and 2) to detect Td-CTLP and MMP-8 protein levels in the tissue samples of peri-implantitis and periodontitis patients. Materials and Methods: proMMP-8 activation by Td-CTLP was analyzed by immunoblots. Tissue specimens were collected from 38 systemically healthy and non-smoking patients; 14 of whom had moderate to severe periodontitis, 10 of whom were suffering from peri-implantitis, and finally 14 of whom showed no sign of periodontal inflammation nor radiological bone decay (control group). The immune-expression levels of MMP-8 and Td-CTLP in the epithelium and the connective tissue were analyzed immunohistochemically. A pixel color-intensity analyze was performed with ImageJ software (version 1.46c; Rasband WS, National Institutes of Health, Bethesda, MD, USA) to obtain a comparable numeral score for each patient's epithelium and connective tissue MMP-8 and Td-CTLP enzyme level. Results: Td-CTLP activated proMMP-8 in vitro by converting the 70−75 kDa proMMP-8 to 65 kDa active MMP-8. Also, lower molecular size 25−50 kDa parts of MMP-8 were formed. There was no statistically significant difference between the study groups in terms of their MMP-8 and Td-CTLP levels in the epithelium or in the connective tissue. Conclusion: Regarding the limits of this study, it can thus be said that the Td-CTLP enzyme can activate the host proMMP-8 enzyme. Tissue protein levels of MMP-8 and Td-CTLP do not seem to be changed in peri-implantitis and in periodontitis.