Gingival levels of monocyte chemoattractant protein-1 (MCP-1) in diabetes mellitus and periodontitis: an experimental study in rats

Sakalhoglu E. E., Ayas B., Luetfloglu M., KELEŞ G., Acikgoez G., Firath E.

CLINICAL ORAL INVESTIGATIONS, cilt.12, sa.1, ss.83-89, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 1
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1007/s00784-007-0148-6
  • Dergi Adı: CLINICAL ORAL INVESTIGATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.83-89
  • Anahtar Kelimeler: MCP-1, diabetes mellitus, periodontitis, gingival inflammation, alveolar bone loss, ALVEOLAR BONE LOSS, CREVICULAR FLUID, AGGRESSIVE PERIODONTITIS, MONONUCLEAR PHAGOCYTES, EXPRESSION, DISEASE, CELLS, RESORPTION, CHEMOKINES, RECRUITMENT
  • İstanbul Üniversitesi Adresli: Evet

Özet

The objectives of this study were to investigate and compare the monocyte chemoattractant protein-1 (MCP-1) levels of gingival tissues in diabetes mellitus (DM) and periodontitis and to reveal the effects of MCP-1 on periodontal inflammation and destruction in these diseases. DM was created in 15 rats (group 1) by streptozotocin injection, and periodontitis was obtained by ligature induction in 15 rats (group 2). Fifteen systemically and periodontally healthy rats were used as control (group 3). Gingival MCP-1 levels were measured by enzyme-linked immunosorbent assay (ELISA). Periodontal inflammation was quantified by the inflammatory cell infiltration in the gingival samples, whereas periodontal destruction was assessed by the alveolar bone loss in the experimental regions. MCP-1 concentrations were higher in groups I and 2 than in group 3 (p<0.001). Increased gingival inflammatory cell infiltration and alveolar bone loss were observed in groups I and 2 compared to group 3 (p<0.001). There were positive correlations among the MCP-1 level, gingival inflammatory cell infiltration, and alveolar bone loss in groups 1 and 2 (p < 0.001). Our results suggest that (1) DM may lead to enhanced MCP-1 production in periodontal tissues likewise for periodontitis and (2) there may be a positive correlation between the MCP-1 concentration and diseased nature of periodontium in both diseases.