PER-1- and OXA-10-like beta-lactamases in ceftazidime-resistant Pseudomonas aeruginosa isolates from intensive care unit patients in Istanbul, Turkey


Aktas Z., Poirel L., Salcioglu M., Ozcan P., Midilli K., Bal C., ...Daha Fazla

CLINICAL MICROBIOLOGY AND INFECTION, cilt.11, sa.3, ss.193-198, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 3
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1111/j.1469-0691.2004.01067.x
  • Dergi Adı: CLINICAL MICROBIOLOGY AND INFECTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.193-198
  • Anahtar Kelimeler: beta-lactamase, ceftazidime resistance, intensive care unit, OXA-10, PER-1, Pseudomonas aeruginosa, EXTENDED-SPECTRUM VARIANT, MOLECULAR EPIDEMIOLOGY, SALMONELLA-TYPHIMURIUM, CLINICAL ISOLATE, ACINETOBACTER, CEPHALOSPORINS, COLONIZATION, PREVALENCE, SEQUENCE, GENE
  • İstanbul Üniversitesi Adresli: Evet

Özet

The presence of PER-1- and OXA-10-like beta-lactamases was investigated by PCR in 49 ceftazidime-resistant Pseudomonas aeruginosa isolates from patients hospitalised in the 24-bed general intensive care unit of the Istanbul Faculty of Medicine during a 12-month period between February 1999 and February 2000. The clonal relatedness of the isolates was investigated by random amplified polymorphic DNA (RAPD) analysis, and the sequences of the PER-1 and OXA genes from all isolates were determined. The rates of resistance of the isolates to imipenem, aztreonam and cefepime were 98%, 92% and 96%, respectively, and to piperacillin and piperacillin-tazobactam were 41% and 37%, respectively. Using the double-disk synergy test, 37% (18/49) of the isolates were identified as extended-spectrum beta-lactamase producers. The PER-1 gene was identified in 86% (42/49) and the OXA-10 gene in 55% (27/49) of the ceftazidime-resistant isolates. Of isolates carrying the PER-1 gene, 48% (20/42) also carried the OXA-10 gene. The respective nucleotide sequences were identical for each isolate. Sixteen RAPD patterns were detected among the PER-1-positive isolates, but 60% (25/42) of the PER-1-positive isolates belonged to two distinct patterns, while the remainder exhibited a wide clonal diversity. The results indicated that the prevalence of PER-1- and OXA-10-like beta-lactamases remains high among ceftazidime-resistant P. aeruginosa isolates in Turkey.